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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PAB14106 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14106, RRID:AB_10548892
- Product name
- ASH2L polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant ASH2L.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cloning and characterization of ASH2L and Ash2l, human and mouse homologs of the Drosophila ash2 gene.
Ikegawa S, Isomura M, Koshizuka Y, Nakamura Y
Cytogenetics and cell genetics 1999;84(3-4):167-72
Cytogenetics and cell genetics 1999;84(3-4):167-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed using ASH2L polyclonal antibody (Cat # PAB14106) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.The antibody recognizes ectopically expressed FLAG-tagged ASH2L (ASH2L-F) in extracts (Lane 2) and a protein of correct size in nuclear extracts from HeLa cells (Lane 1).On the left side, a molecular weight marker is shown.The panel A shows immunostaining using ASH2L polyclonal antibody (Cat # PAB14106), the panel B is the immunostaining of the same western blot with anti-FLAG antibodies.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed on HeLa nuclear extract (HeLa NE, 20 ug) using ASH2L polyclonal antibody (Cat # PAB14106) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.The antibody recognizes endogenous human ASH2 in HeLa nuclear extract.On the left side, a molecular weight marker is shown.The arrow indicates the location of the protein of interest.
