Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12331-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASH2L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: HGYPLEHPF NKDGYRYILA EPDPHAPDPE KLELDCWAGK PIPGDLYRAC LYERVLLALH DRAPQLKISD DRLTVVGEKG YSMVRASHGV RKGAWYFEIT VDEMPPDTAA RLGWSQPLGN LQAPLGYDKF SYSWRSKKGT KFHQSIGKHY SSGYGQGDVL GFYINLPEDT ETAKSLPDTY KDKALIKFKS YLYFEEKDFV DKAEKSLKQT PHSEIIFYKN GVNQGVAYKD IFEGVYFPAI SLYKSCTVSI NFGPCFKYPP KDLTYRPMSD MGWGAVVEHT LADVLYHVET EVDGRRSPPW EP (329-628 aa encoded by BC015936)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COS-7 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12331-1-AP ( ASH2L antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using (Product # 12331-1-AP) (ASH2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ASH2L (IP:12331-1-AP, 4ug; Detection:12331-1-AP 1:500) with COLO 320 cells lysate 2000ug.
