UM800059

antibody from OriGene

Targeting: RRM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

UM800059

Provider

OriGene

Proper citation

OriGene Cat#UM800059, RRID:AB_2629169

Product name

RRM1 mouse monoclonal antibody,clone UMAB165

Antibody type

Monoclonal

Description

RRM1 mouse monoclonal antibody,clone UMAB165

Host

Mouse

Conjugate

Unconjugated

Epitope

RRM1

Isotype

IgG

Antibody clone number

UMAB165

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-RRM1 mouse monoclonal antibody (UM800059, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RRM1 mouse monoclonal antibody. (UM800059; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RRM1 mouse monoclonal antibody. (UM800059; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RRM1 mouse monoclonal antibody. (UM800059; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RRM1 mouse monoclonal antibody. (UM800059; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Validation comment

IHC