Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB4017 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4017, RRID:AB_1578639
- Product name
- PFKFB3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PFKFB3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The human ubiquitous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene (PFKFB3): promoter characterization and genomic structure.
An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect.
Characterization of a human placental fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase.
Navarro-Sabaté A, Manzano A, Riera L, Rosa JL, Ventura F, Bartrons R
Gene 2001 Feb 7;264(1):131-8
Gene 2001 Feb 7;264(1):131-8
An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect.
Chesney J, Mitchell R, Benigni F, Bacher M, Spiegel L, Al-Abed Y, Han JH, Metz C, Bucala R
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 16;96(6):3047-52
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 16;96(6):3047-52
Characterization of a human placental fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase.
Sakakibara R, Kato M, Okamura N, Nakagawa T, Komada Y, Tominaga N, Shimojo M, Fukasawa M
Journal of biochemistry 1997 Jul;122(1):122-8
Journal of biochemistry 1997 Jul;122(1):122-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PFKFB3 polyclonal antibody (Cat # PAB4017) is used in Western blot to detect PFKFB3 in Jurkat cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PFKFB3 polyclonal antibody (Cat # PAB4017) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)