HPA008266

antibody from Atlas Antibodies

Targeting: PFKFB3

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA008266

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008266, RRID:AB_1855239

Product name

Anti-PFKFB3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDAT
NTTRERRHMILHFAKENDFKAFFIESVCDDPTVVA
SNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEAS
YQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQD


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line A-549 and human cell line CACO-2.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and PFKFB3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401447).

Sample type

HUMAN

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Sample type

HUMAN