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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF5935 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse MBD4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse MBD4 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O95243- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse MBD4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MBD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5935) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MBD4 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
