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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-22017 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNase I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4.
- Concentration
- 1.19 mg/mL
Submitted references Role of Bacterial and Host DNases on Host-Pathogen Interaction during Streptococcus suis Meningitis.
Meurer M, Öhlmann S, Bonilla MC, Valentin-Weigand P, Beineke A, Hennig-Pauka I, Schwerk C, Schroten H, Baums CG, Köckritz-Blickwede MV, de Buhr N
International journal of molecular sciences 2020 Jul 25;21(15)
International journal of molecular sciences 2020 Jul 25;21(15)
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNase I using 30 µg of MOLT4 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a DNase I polyclonal antibody (Product # PA5-22017) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNase I was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNase I Polyclonal Antibody (Product # PA5-22017) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DNase I was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: DNase I Polyclonal Antibody (Product # PA5 22017) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNase I Polyclonal Antibody detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. DNase I Polyclonal Antibody (Product # PA5-22017) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNase I Polyclonal Antibody detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. DNase I Polyclonal Antibody (Product # PA5-22017) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Host DNases are detectable and active during S. suis meningitis in choroid plexus, CSF and serum. ( A ) By immunofluorescence staining, we could show that the amount of DNase 1 in the choroid plexus is different in animals with inflammation, compared to animals without inflammation of the choroid plexus. In the choroid plexus of an infected pig without clinical signs of CNS disorders only weak DNase 1 signals were detected in the early phase experiment. In pigs with clinical signs of CNS disorders a high DNase 1 signal in the region of the choroid plexus epithelial cells was observed. PR-39 signal, as a marker for neutrophils, was found in these pigs as well (blue = DNA (Hoechst); green = PR-39; red = DNase 1 (scale bar upper panel = 50 um; lower panel = 20 um; arrows mark transmigrating or already transmigrated PR-39 positive neutrophils). Activity of DNases was observed in serum ( B ) and in CSF ( C ) of all pigs of the early phase experiment. A significant increase of DNase activity post-infection was found in serum for both infection groups after 48 h and for the S. suis 10-infected group also after 96 h. In CSF after 96 h, a higher increase was observed than after 48 h in both infection groups. Data shown as mean +- SD. Statistical analysis: unpaired Mann-Whitney test (* p < 0.05, ** p < 0.01).
