MAB1354-100

antibody from R&D Systems

Targeting: ITGA4 CD49D

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1354-100

Provider

R&D Systems

Product name

Human Integrin alpha 4/CD49d Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Integrin alpha 4/CD49d. Recognizes the human Integrin alpha 4/CD49d subunit.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

7.2R

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCζ tumor-suppressive activities and regulating integrin affinity modulation.

Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
Scientific reports 2015 Mar 20;5:9275

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The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.

Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis and rheumatism 2007 Jun;56(6):1817-26

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A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.

Carman CV, Springer TA
The Journal of cell biology 2004 Oct 25;167(2):377-88

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Integrin alpha 4/CD49d in U937 Human Cell Line. Integrin alpha 4/ CD49d was detected in immersion fixed U937 human histiocytic lymphoma cell line using 10 µg/mL Mouse Anti-Human Integrin alpha 4/CD49d Monoclonal Antibody (Catalog # MAB1354) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin alpha 4/CD49d Monoclonal Antibody (Catalog # MAB1354, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.