Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 304310 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#304310, RRID:AB_2130039
- Product name
- LEAF™ Purified anti-human CD49d [9F10]
- Antibody type
- Monoclonal
- Antigen
- CD49d
- Description
- CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells. The 9F10 antibody is able to costimulate T cell activation.
- Reactivity
- Human, Bovine, Canine, Feline, Horse, Sheep, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 1 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Submitted references Uveitis-associated epitopes of retinal antigens are pathogenic in the humanized mouse model of uveitis and identify autoaggressive T cells.
CD49d provides access to "untouched" human Foxp3+ Treg free of contaminating effector cells.
Mattapallil MJ, Silver PB, Mattapallil JJ, Horai R, Karabekian Z, McDowell JH, Chan CC, James EA, Kwok WW, Sen HN, Nussenblatt RB, David CS, Caspi RR
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 15;187(4):1977-85
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 15;187(4):1977-85
CD49d provides access to "untouched" human Foxp3+ Treg free of contaminating effector cells.
Kleinewietfeld M, Starke M, Di Mitri D, Borsellino G, Battistini L, Rötzschke O, Falk K
Blood 2009 Jan 22;113(4):827-36
Blood 2009 Jan 22;113(4):827-36
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained with LEAF™purified 9F10, followed by anti-mouse IgGs FITC
- Conjugate
- Unconjugated