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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [2]
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- Product number
- PA5-20599 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITGA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Despite its predicted molecular weight, Integrin alpha4 often migrates at a higher molecular weight in SDS-PAGE. PA5-20599 can be used with blocking peptide PEP-0719.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Specific phosphorylations transmit signals from leukocyte β2 to β1 integrins and regulate adhesion.
Uotila LM, Jahan F, Soto Hinojosa L, Melandri E, Grönholm M, Gahmberg CG
The Journal of biological chemistry 2014 Nov 14;289(46):32230-32242
The Journal of biological chemistry 2014 Nov 14;289(46):32230-32242
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of (A) 25 and (B) 100 ng of recombinant integrin alpha 4 using a CD49d/Integrin alpha 4 polyclonal antibody (Product # PA5-20599) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Integrin alpha 4 using (A) 25 and (B) 100 ng of recombinant Integrin alpha 4 with ITGA4 Polyclonal Antibody (Product # PA5-20599) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Jurkat cells using a CD49d/Integrin alpha 4 polyclonal antibody (Product # PA5-20599) at a 2 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Integrin alpha 4 in Jurkat cells with ITGA4 Polyclonal Antibody (Product # PA5-20599) at 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
