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antibody from Novus Biologicals
Targeting: GPER1
CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NLS4271 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NLS4271, RRID:AB_2263520
- Product name
- Rabbit Polyclonal GPER/GPR30 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Sex-specific estrogen regulation of hypothalamic astrocyte estrogen receptor expression and glycogen metabolism in rats.
Impact of caudal hindbrain glycogen metabolism on A2 noradrenergic neuron AMPK activation and ventromedial hypothalamic nucleus norepinephrine activity and glucoregulatory neurotransmitter marker protein expression.
Sex-dimorphic estrogen receptor regulation of ventromedial hypothalamic nucleus glucoregulatory neuron adrenergic receptor expression in hypoglycemic male and female rats.
Norepinephrine control of ventromedial hypothalamic nucleus glucoregulatory neurotransmitter expression in the female rat: Role of monocarboxylate transporter function.
GPER and ERα expression in abnormal endometrial proliferations.
Estradiol accelerates the effects of fluoxetine on serotonin 1A receptor signaling.
Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer.
GPR30 is necessary for estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the rat hypothalamus.
G-1-activated membrane estrogen receptors mediate increased contractility of the human myometrium.
Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells.
17Beta-estradiol at low concentrations acts through distinct pathways in normal versus benign prostatic hyperplasia-derived prostate stromal cells.
Differential regulation of dopamine transporter function and location by low concentrations of environmental estrogens and 17beta-estradiol.
Nongenomic mechanisms of physiological estrogen-mediated dopamine efflux.
The roles of membrane estrogen receptor subtypes in modulating dopamine transporters in PC-12 cells.
Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP
Molecular and cellular endocrinology 2020 Mar 15;504:110703
Molecular and cellular endocrinology 2020 Mar 15;504:110703
Impact of caudal hindbrain glycogen metabolism on A2 noradrenergic neuron AMPK activation and ventromedial hypothalamic nucleus norepinephrine activity and glucoregulatory neurotransmitter marker protein expression.
Alshamrani AA, Bheemanapally K, Ibrahim MMH, Briski KP
Neuropeptides 2020 Aug;82:102055
Neuropeptides 2020 Aug;82:102055
Sex-dimorphic estrogen receptor regulation of ventromedial hypothalamic nucleus glucoregulatory neuron adrenergic receptor expression in hypoglycemic male and female rats.
Uddin MM, Mahmood ASMH, Ibrahim MMH, Briski KP
Brain research 2019 Oct 1;1720:146311
Brain research 2019 Oct 1;1720:146311
Norepinephrine control of ventromedial hypothalamic nucleus glucoregulatory neurotransmitter expression in the female rat: Role of monocarboxylate transporter function.
Hasan Mahmood ASM, Mandal SK, Bheemanapally K, Ibrahim MMH, Briski KP
Molecular and cellular neurosciences 2019 Mar;95:51-58
Molecular and cellular neurosciences 2019 Mar;95:51-58
GPER and ERα expression in abnormal endometrial proliferations.
Tica AA, Tica OS, Georgescu CV, Pirici D, Bogdan M, Ciurea T, Mogoantă SŞ, Georgescu CC, Comănescu AC, Bălşeanu TA, Ciurea RN, Osiac E, Buga AM, Ciurea ME
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2016;57(2):413-8
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2016;57(2):413-8
Estradiol accelerates the effects of fluoxetine on serotonin 1A receptor signaling.
Li Q, Sullivan NR, McAllister CE, Van de Kar LD, Muma NA
Psychoneuroendocrinology 2013 Jul;38(7):1145-57
Psychoneuroendocrinology 2013 Jul;38(7):1145-57
Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer.
Jala VR, Radde BN, Haribabu B, Klinge CM
BMC cancer 2012 Dec 28;12:624
BMC cancer 2012 Dec 28;12:624
GPR30 is necessary for estradiol-induced desensitization of 5-HT1A receptor signaling in the paraventricular nucleus of the rat hypothalamus.
McAllister CE, Creech RD, Kimball PA, Muma NA, Li Q
Psychoneuroendocrinology 2012 Aug;37(8):1248-60
Psychoneuroendocrinology 2012 Aug;37(8):1248-60
G-1-activated membrane estrogen receptors mediate increased contractility of the human myometrium.
Maiti K, Paul JW, Read M, Chan EC, Riley SC, Nahar P, Smith R
Endocrinology 2011 Jun;152(6):2448-55
Endocrinology 2011 Jun;152(6):2448-55
Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells.
Jeng YJ, Kochukov M, Watson CS
Environmental health : a global access science source 2010 Oct 15;9:61
Environmental health : a global access science source 2010 Oct 15;9:61
17Beta-estradiol at low concentrations acts through distinct pathways in normal versus benign prostatic hyperplasia-derived prostate stromal cells.
Park II, Zhang Q, Liu V, Kozlowski JM, Zhang J, Lee C
Endocrinology 2009 Oct;150(10):4594-605
Endocrinology 2009 Oct;150(10):4594-605
Differential regulation of dopamine transporter function and location by low concentrations of environmental estrogens and 17beta-estradiol.
Alyea RA, Watson CS
Environmental health perspectives 2009 May;117(5):778-83
Environmental health perspectives 2009 May;117(5):778-83
Nongenomic mechanisms of physiological estrogen-mediated dopamine efflux.
Alyea RA, Watson CS
BMC neuroscience 2009 Jun 16;10:59
BMC neuroscience 2009 Jun 16;10:59
The roles of membrane estrogen receptor subtypes in modulating dopamine transporters in PC-12 cells.
Alyea RA, Laurence SE, Kim SH, Katzenellenbogen BS, Katzenellenbogen JA, Watson CS
Journal of neurochemistry 2008 Aug;106(4):1525-33
Journal of neurochemistry 2008 Aug;106(4):1525-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with rat hypothalamus 5-10ug protein loaded per lane. Image courtesy provided by Carrie McAllister.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GPER/GPR30 Antibody [NLS4271] - Direct association of dopamine transporter and estrogen receptors on the plasma membrane vs. in total protein. Co-immunoprecipitation of dopamine transporter after 9 min 10-9 M E2 treatment and subsequent probing with antibodies for DAT, ERalpha, ERbeta, and GPR30. This is a representative immunoblot of 3 experiments Image collected and cropped by CiteAb from the following publication (http://www.biomedcentral.com/1471-2202/10/59), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with human brain, hippocampus.
