PA5-89291

antibody from Invitrogen Antibodies

Targeting: MAOA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89291

Provider

Invitrogen Antibodies

Product name

MAOA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MENQEKASIA GHMFDVVVIG GGISGLSAAK LLTEYGVSVL VLEARDRVGG RTYTIRNEHV DYVDVGGAYV GPTQNRILRL SKELGIETYK VNVSERLVQY VKGKTYPFRG AFPPVWNPIA YLDYNNLWRT IDNMGKEIPT DAPWEAQHAD KWDKMTMKEL IDKICWTKTA RRFAYLFVNI NVTSEPHEVS ALWFLWYVKQ CGGTTRIFSV TNGGQERKFV GGSGQVSERI MDLLGDQVKL NHPVTHVDQS SDNIIIETLN; Positive Samples: A-549, PC-3, HeLa, HT-29, Mouse lung, Mouse intestine, Mouse kidney, Mouse liver, NCI-H460; Cellular Location: Cytoplasmic side, Mitochondrion outer membrane, Single-pass type IV membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MAOA in extracts of various cell lines using MAOA Polyclonal Antibody (Product # PA5-89291) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MAOA in extracts of various cell lines using MAOA Polyclonal Antibody (Product # PA5-89291) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MAOA was performed in C6 cells using MAOA Polyclonal Antibody (Product # PA5-89291).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MAOA was performed in NIH/3T3 cells using MAOA Polyclonal Antibody (Product # PA5-89291).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MAOA in paraffin-embedded mouse brain using MAOA Polyclonal Antibody (Product # PA5-89291) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MAOA in paraffin-embedded rat brain using MAOA Polyclonal Antibody (Product # PA5-89291) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MAOA in paraffin-embedded human thyroid cancer using MAOA Polyclonal Antibody (Product # PA5-89291) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MAOA in paraffin-embedded mouse brain using MAOA Polyclonal Antibody (Product # PA5-89291) at a dilution of 1:100.