PA5-118660

antibody from Invitrogen Antibodies

Targeting: PRKCQ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118660

Provider

Invitrogen Antibodies

Product name

Phospho-PKC theta (Tyr90) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PKC theta (Tyr90) in Jurkat cells using a Phospho-PKC theta (Tyr90) polyclonal antibody (Product # PA5-118660). The -/+ symbols identify the absence or presence of N peptide (non-phospho peptide) and P peptide(phospho peptide).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PKC theta (Tyr90) in Hepg2 cells using a Phospho-PKC theta (Tyr90) polyclonal antibody (Product # PA5-118660). Lane 1 was treated with the blocking peptide.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Phospho-PKC theta (Tyr90) in Hela cells using a Phospho-PKC theta (Tyr90) polyclonal antibody (Product # PA5-118660). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1:600, was used as secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Phospho-PKC theta (Tyr90) in Hela cells using a Phospho-PKC theta (Tyr90) polyclonal antibody (Product # PA5-118660). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1:600, was used as secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-PKC theta (Tyr90) in Human lung cancer tissue using a Phospho-PKC theta (Tyr90) polyclonal antibody (Product # PA5-118660). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-PKC theta (Tyr90) in Rat gastric tissue using a Phospho-PKC theta (Tyr90) polyclonal antibody (Product # PA5-118660). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-PKC theta (Tyr90) in Mouse testis tissue using a Phospho-PKC theta (Tyr90) polyclonal antibody (Product # PA5-118660). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.