AF4368

antibody from R&D Systems

Targeting: PRKCQ

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4368

Provider

R&D Systems

Product name

Human/Mouse PKC theta Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human/mouse PKC theta .

Reactivity

Human, Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q04759


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human PKC theta by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKC theta at approximately 82 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse PKC theta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4368) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human PKC theta by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PKC theta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4368) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC theta at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.