H00005026-M01

P2RX5 antibody from Novus Biologicals

LRH-1, P2X5

Western blot

Supportive data in Antibodypedia

ELISA

Supportive data in Antibodypedia

Blocking/Neutralizing

Data presented on provider website

Provider product page for H00005026-M01

Antibody Data

Product number

H00005026-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00005026-M01, RRID:AB_546715

Product name

Mouse Monoclonal P2X5/P2RX5 Antibody

Provider product page

Antibody type

Monoclonal

Description

IgG purified. P2RX5 - purinergic receptor P2X, ligand-gated ion channel, 5

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.
Abramowski P, Ogrodowczyk C, Martin R, Pongs O
PloS one 2014;9(9):e104692

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 over-expressed 293 cell line, cotransfected with P2RX5 Validated Chimera RNAi ( Cat # H00005026-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with P2RX5 monoclonal antibody (M01), clone 1C5 (Cat # H00005026-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 expression in transfected 293T cell line by P2RX5 monoclonal antibody (M01), clone 1C5.Lane 1: P2RX5 transfected lysate(46.42 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Detection limit for recombinant GST tagged P2RX5 is approximately 1ng/ml as a capture antibody.