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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28838 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2X5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, HeLa, HepG2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using P2X5 Polyclonal Antibody (Product # PA5-28838). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. P2X5 Polyclonal Antibody (Product # PA5-28838) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3), IM-9 (Lane 4) and DU 145 (Lane 5). The blot was probed with Anti-P2X5 Polyclonal Antibody (Product # PA5-28838, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 52 kDa band corresponding to P2X5 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of P2X5 in methanol-fixed A431 cells using a P2X5 polyclonal antibody (Product # PA5-28838) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using P2X5 (Product # PA5-28838) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
