PA5-28838

P2RX5 antibody from Invitrogen Antibodies

LRH-1, P2X5

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Data presented on provider website

Provider product page for PA5-28838

Antibody Data

Product number

PA5-28838

Provider

Invitrogen Antibodies

Product name

Anti-P2X5 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, HeLa, HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P2X5 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a P2X5 polyclonal antibody (Product # PA5-28838) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3), IM-9 (Lane 4) and DU 145 (Lane 5). The blot was probed with Anti-P2X5 Polyclonal Antibody (Product # PA5-28838, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 52 kDa band corresponding to P2X5 was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of P2X5 in methanol-fixed A431 cells using a P2X5 polyclonal antibody (Product # PA5-28838) at a 1:200 dilution.