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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF3499a - Provider product page
- Provider
- Abgent
- Product name
- ZIC2 Antibody (C-Term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide-affinity purified goat antibody
- Reactivity
- Mouse, Rat, Bovine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1mg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -1169°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
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- Abgent (provider)
- Main image
- Experimental details
- RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with AF3499a (0.5ug/ml) in the right panel and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel. Mock-transfected RWPE1 in lanes 1 and expressing GFP in lanes 2. Primary incubations were overnight at 4C. Detected by chemiluminescence.
