Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-28171 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-28171 detects Aquaporin Adipose Specific from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Aquaporin 7 Polyclonal Antibody (Product # PA1-28171) and a 80 kDa band corresponding to Aquaporin 7 was observed in 3T3-L1 differentiated to adipocytes and Mouse Brown Adipose tissue and not in 3T3-L1, Mouse Adipose tissue, Mouse Brain and Mouse Skeletal Muscle which are reported negative for Aquaporin 7 expression. Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to Adipocytes (Lane 2), tissue extracts (30 µg lysate) of Mouse Brown Adipose tissue (Lane 3), Mouse Adipose tissue (Lane 4), Mouse Brain (Lane 5) and Mouse Skeletal Muscle (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).