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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- GTX115082 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115082, RRID:AB_10619797
- Product name
- Transgelin 2 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references SB-T-121205, a next-generation taxane, enhances apoptosis and inhibits migration/invasion in MCF-7/PTX cells.
Salvianolic acid A reverses paclitaxel resistance in human breast cancer MCF-7 cells via targeting the expression of transgelin 2 and attenuating PI3 K/Akt pathway
Paeonol reverses paclitaxel resistance in human breast cancer cells by regulating the expression of transgelin 2.
Proteomic analysis of the proteins that are associated with the resistance to paclitaxel in human breast cancer cells.
Zheng X, Wang C, Xing Y, Chen S, Meng T, You H, Ojima I, Dong Y
International journal of oncology 2017 Mar;50(3):893-902
International journal of oncology 2017 Mar;50(3):893-902
Salvianolic acid A reverses paclitaxel resistance in human breast cancer MCF-7 cells via targeting the expression of transgelin 2 and attenuating PI3 K/Akt pathway
Cai J, Chen S, Zhang W, Zheng X, Hu S, Pang C, Lu J, Xing J, Dong Y
Phytomedicine 2014 October;21(12):1725-1732
Phytomedicine 2014 October;21(12):1725-1732
Paeonol reverses paclitaxel resistance in human breast cancer cells by regulating the expression of transgelin 2.
Cai J, Chen S, Zhang W, Hu S, Lu J, Xing J, Dong Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2014 Jun 15;21(7):984-91
Phytomedicine : international journal of phytotherapy and phytopharmacology 2014 Jun 15;21(7):984-91
Proteomic analysis of the proteins that are associated with the resistance to paclitaxel in human breast cancer cells.
Chen S, Dong Q, Hu S, Cai J, Zhang W, Sun J, Wang T, Xie J, He H, Xing J, Lu J, Dong Y
Molecular bioSystems 2014 Feb;10(2):294-303
Molecular bioSystems 2014 Feb;10(2):294-303
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Supportive validation
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- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX115082 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: A549 B: HeLa 12% SDS PAGE GTX115082 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Experimental details
- Transgelin 2 antibody detects Transgelin 2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody (GTX115082) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in ice-cold MeOH for 5 min.Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
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- GeneTex (provider)
- Main image
- Experimental details
- Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
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- Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
