60020

antibody from STEMCELL Technologies Inc

Targeting: ITGA2 CD49B

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

60020

Provider

STEMCELL Technologies Inc

Product name

Anti-Mouse CD49b Antibody, Clone DX5

Antibody type

Monoclonal

Description

The DX5 antibody reacts with murine CD49b (integrin ?2), an ~150 kDa type 1 transmembrane glycoprotein that associates non-covalently with CD29 (integrin ?1) to form the heterodimeric CD49b/CD29 complex known as VLA-2, a receptor for extracellular matrix proteins such as collagen, E-cadherin, fibronectin, and laminin. CD49b is highly expressed by platelets and is found on a majority of NK cells, on NKT cells, and on a small subset of CD8+ T cells; the latter population increases substantially following viral infection. CD49b is also expressed by several tissues, including intestine, kidney, mammary gland, and lung. The DX5 antibody is particularly useful for identifying NK cells in mice lacking the NK1.1 antigen. Binding of the DX5 antibody has not been observed to affect the function of the VLA-2 integrin. DX5 binding is, however, blocked by the clone HM?2 antibody. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ Mouse NK Cell Enrichment Kit (Catalog #19755).

Reactivity

Mouse

Host

Mouse

Isotype

IgM

Vial size

500 µg

Storage

2 - 8°C