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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB8076 - Provider product page
- Provider
- R&D Systems
- Product name
- Human P-Rex1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human P-Rex1 in ELISAs and Western Blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8TCU6- Isotype
- IgG
- Antibody clone number
- 869704
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human P-Rex1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for P-Rex1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human P-Rex1 by Simple WesternTM. Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for P-Rex1 at approximately 175 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human P-Rex1 Monoclonal Antibody (Catalog # MAB8076) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
