NBP1-36727

antibody from Novus Biologicals

Targeting: SULF2 HSULF-2, KIAA1247, SULF-2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-36727

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-36727, RRID:AB_2197470

Product name

Mouse Monoclonal Sulfatase-2/SULF2 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Does not recognize human or mouse Sulfatase 1 or other family members.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Epithelial Deletion of Sulf2 Exacerbates Bleomycin-Induced Lung Injury, Inflammation, and Mortality.

Yue X
American journal of respiratory cell and molecular biology 2017 Nov;57(5):560-569

---

Sulfatase 2 promotes breast cancer progression through regulating some tumor-related factors.

Zhu C, He L, Zhou X, Nie X, Gu Y
Oncology reports 2016 Mar;35(3):1318-28

---

MYCN-dependent expression of sulfatase-2 regulates neuroblastoma cell survival.

Solari V, Borriello L, Turcatel G, Shimada H, Sposto R, Fernandez GE, Asgharzadeh S, Yates EA, Turnbull JE, DeClerck YA
Cancer research 2014 Nov 1;74(21):5999-6009

---

Overexpression of Sulf2 in idiopathic pulmonary fibrosis.

Yue X, Lu J, Auduong L, Sides MD, Lasky JA
Glycobiology 2013 Jun;23(6):709-19

---

SULF2 expression by immunohistochemistry and overall survival in oesophageal cancer: a cohort study.

Lui NS, van Zante A, Rosen SD, Jablons DM, Lemjabbar-Alaoui H
BMJ open 2012;2(6)

---

Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis.

Lemjabbar-Alaoui H, van Zante A, Singer MS, Xue Q, Wang YQ, Tsay D, He B, Jablons DM, Rosen SD
Oncogene 2010 Feb 4;29(5):635-46

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis showing Sulfatase 2 expression in MCF7 conditioned media (CM). In CM, the 75 kDa band is dominant (Tang R, Rosen SD. J Biol Chem. 2009 Aug 7;284(32):21505-14). Image courtesy of Mark Singer at UCSF.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Immunohistochemical analysis of Sulfatase 2 in non-small-cell lung carcinoma. (left) lung squamous cell carcinoma stained with 2B4 antibody and (right) tumor-associated stromal cells stained with 2B4 antibody. Image courtesy of Mark Singer at UCSF.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis using the FITC conjugate of NBP1-36727. Staining of Human lung cancer line with Sulfatase2-FITC. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - An intracellular stain was performed on HeLa cells with Sulfatase-2/SULF2 Antibody (2B4) NBP1-36727AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.