Explore
Validate
LearnMAB765-100
antibody from R&D Systems
Targeting: FGFR1
BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB765-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGFR1 (IIIb) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects IIIb isoforms of human FGF R1. In direct ELISAs and Western blots, this antibody detected IIIb but not IIIc isoforms of human FGF R1, human FGF R2, and mouse FGF R2. In direct ELISA, approximately 20% cross-reactivity was observed with recombinant human (rh) FGF R3 (IIIb). No cross-reactivity was observed with rhFGF R4 or with IIIIc isoforms of rhFGF R1, rhFGF R2, rmFGF R2, or rhFGF R3.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 133111
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
Stem cells (Dayton, Ohio) 2012 Jul;30(7):1455-64
Stem cells (Dayton, Ohio) 2012 Jul;30(7):1455-64
Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM
The Journal of biological chemistry 2009 Jun 12;284(24):16693-703
The Journal of biological chemistry 2009 Jun 12;284(24):16693-703
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FGF R1 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody. Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND50 is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL).
