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LearnF50611
antibody from NSJ Bioreagents
Targeting: FGFR1
BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F50611 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- FGFR1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 19-48 from the human protein was used as the immunogen for this FGFR1 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the FGFR1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- The FGFR1 antibody used in western blot to detect FGFR1 in NIH3T3 cell lysate. Predicted molecular weight: 75-160 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF: Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 7E3 and FGFR1 antibody. FGFR is visualized as red fluorescence (i,iv,vii), A1B3 is visualized as green fluorescence (ii,v,viii), and colocalization of FGF2 and fibrinogen receptors is shown as yellow fluorescence (iii,vi,ix). Insets represent the background fluorescence.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma tissue stained with the FGFR1 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using FGFR1 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
