MAB669-100

antibody from R&D Systems

Targeting: FST FS

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB669-100

Provider

R&D Systems

Product name

Human Follistatin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human Follistatin in direct ELISAs and Western blots. It recognizes the 288 aa, 300 aa, and 315 aa isoforms of human Follistatin. In direct ELISAs and Western blots, this antibody shows approximately 50% cross-reactivity with recombinant mouse Follistatin.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P19883


Isotype

IgG

Antibody clone number

85918

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro.

Wu W, Wu XL, Ji YQ, Gao Z
Molecular medicine reports 2017 Jul;16(1):95-100

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BMP-driven NRF2 activation in esophageal basal cell differentiation and eosinophilic esophagitis.

Jiang M, Ku WY, Zhou Z, Dellon ES, Falk GW, Nakagawa H, Wang ML, Liu K, Wang J, Katzka DA, Peters JH, Lan X, Que J
The Journal of clinical investigation 2015 Apr;125(4):1557-68

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Nucleolar follistatin promotes cancer cell survival under glucose-deprived conditions through inhibiting cellular rRNA synthesis.

Gao X, Wei S, Lai K, Sheng J, Su J, Zhu J, Dong H, Hu H, Xu Z
The Journal of biological chemistry 2010 Nov 19;285(47):36857-64

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Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells.

Kobayashi T, Iwasaki T, Amagai M, Ohyama M
The Journal of investigative dermatology 2010 Aug;130(8):1988-95

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Thapsigargin resistance in human prostate cancer cells.

O'Neill JP, Velalar CN, Lee DI, Zhang B, Nakanishi T, Tang Y, Selaru F, Ross D, Meltzer SJ, Hussain A
Cancer 2006 Aug 1;107(3):649-59

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Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts.

Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J
Arthritis and rheumatism 2004 Aug;50(8):2521-30

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Follistatin in Human Pituitary. Follistatin was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Follistatin Monoclonal Antibody (Catalog # MAB669) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.