Antibody data
Interleukin-4 suppresses the cytotoxic potential of in vitro generated, adaptive regulatory CD4 T cells by down-regulation of granzyme B.
Bratke K, Goettsching H, Kuepper M, Geyer S, Luttmann W, Virchow JC
Immunology 2009 Jul;127(3):338-44
Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood.
Bratke K, Kuepper M, Bade B, Virchow JC Jr, Luttmann W
European journal of immunology 2005 Sep;35(9):2608-16
Supportive validation
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Acris Antibodies GmbH (provider)
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- Experimental details
- Fig.1: FACS analysis of BOSC23 cells using GM-24C3 DM1209. BOSC23 cells were transiently transfected with anexpression vector encoding either Granzyme K (red curve) oran irrelevant protein (control transfectant: black curve).Binding of GM-24C3 was detected with a PE-conjugatedsecondary antibody. A positive signal was obtained only withGranzyme K transfected cells.
Supportive validation
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Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fig.2: Intracellular detection of granzyme B in human PBMC.FACS analysis of human PBMC using hybridoma supernatantof GM-24C3. PBMC were cultivated in the presence ofphorbolester and ionomycin subsequently fixed andpermeabilised. Binding of GM-24C3 was detected with aFITC-conjugated secondary antibody.
Supportive validation
- Submitted by
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Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fig3: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an anti-myc monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control(black curves). For specificity testing, GM-24C3 hybridoma supernatant was tested on all transfectants. A positive signal was obtained only with Granzyme K transfected cells (red curves).
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