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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-36488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PINK1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Human PINK1 protein sequence (between residues 100-250), only reactive to isoform 1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references p66shc siRNA-Encapsulated PLGA Nanoparticles Ameliorate Neuropathic Pain Following Spinal Nerve Ligation.
Chalcomoracin is a potent anticancer agent acting through triggering Oxidative stress via a mitophagy- and paraptosis-dependent mechanism.
TLR4-mediated autophagic impairment contributes to neuropathic pain in chronic constriction injury mice.
Delivery of exogenous mitochondria via centrifugation enhances cellular metabolic function.
Novel Role of the Mitochondrial Protein Fus1 in Protection from Premature Hearing Loss via Regulation of Oxidative Stress and Nutrient and Energy Sensing Pathways in the Inner Ear.
PPAR-γ activation attenuates deltamethrin-induced apoptosis by regulating cytosolic PINK1 and inhibiting mitochondrial dysfunction.
Shin N, Shin HJ, Yi Y, Beom J, Lee W, Lee CH, Kim DW
Polymers 2020 Apr 29;12(5)
Polymers 2020 Apr 29;12(5)
Chalcomoracin is a potent anticancer agent acting through triggering Oxidative stress via a mitophagy- and paraptosis-dependent mechanism.
Han H, Chou CC, Li R, Liu J, Zhang L, Zhu W, Hu J, Yang B, Tian J
Scientific reports 2018 Jun 22;8(1):9566
Scientific reports 2018 Jun 22;8(1):9566
TLR4-mediated autophagic impairment contributes to neuropathic pain in chronic constriction injury mice.
Piao Y, Gwon DH, Kang DW, Hwang TW, Shin N, Kwon HH, Shin HJ, Yin Y, Kim JJ, Hong J, Kim HW, Kim Y, Kim SR, Oh SH, Kim DW
Molecular brain 2018 Feb 27;11(1):11
Molecular brain 2018 Feb 27;11(1):11
Delivery of exogenous mitochondria via centrifugation enhances cellular metabolic function.
Kim MJ, Hwang JW, Yun CK, Lee Y, Choi YS
Scientific reports 2018 Feb 20;8(1):3330
Scientific reports 2018 Feb 20;8(1):3330
Novel Role of the Mitochondrial Protein Fus1 in Protection from Premature Hearing Loss via Regulation of Oxidative Stress and Nutrient and Energy Sensing Pathways in the Inner Ear.
Tan WJT, Song L, Graham M, Schettino A, Navaratnam D, Yarbrough WG, Santos-Sacchi J, Ivanova AV
Antioxidants & redox signaling 2017 Sep 10;27(8):489-509
Antioxidants & redox signaling 2017 Sep 10;27(8):489-509
PPAR-γ activation attenuates deltamethrin-induced apoptosis by regulating cytosolic PINK1 and inhibiting mitochondrial dysfunction.
Ko J, Park JH, Park YS, Koh HC
Toxicology letters 2016 Oct 17;260:8-17
Toxicology letters 2016 Oct 17;260:8-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Analysis of (A) Partial Recombinant Human PINK-1 protein with estimated molecular weight at 13kDa and (B) Human Liver lysate using PINK1 antibody clone 8E10.1D6 at 3ug/ml concentration, molecular weight ~64 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Whole cell protein from HeLa cells treated with or without valinomycin (1 uM, 24h) as indicated was separated by SDS-PAGE on a 7.5% polyacrylamide gel. Protein was transferred to PVDF membrane and probed with 2 ug/ml anti-PINK1 in 1% BSA and detected with an HRP-conjugated anti-mouse secondary antibody using chemiluminescence. PINK1 is seen to be upregulated with treament and with a molecular weight at approximately 60 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Analysis of FFPE tissue section of human hepatocellular carcinoma using PINK1 antibody (clone 8E10.1D6) at 5 ug/ml concentration. The cancer cells developed an expected punctate to granular cytoplasmic staining with no signal in the tumor stroma.
