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LearnMAB2585
antibody from R&D Systems
Targeting: CSPG4
CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB2585 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NG2/MCSP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NG2/MCSP in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- LHM-2
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Amyloid-beta 1-40 is associated with alterations in NG2+ pericyte population ex vivo and in vitro.
Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Isolation and characterization of equine native MSC populations.
Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro.
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
Chondroitin sulfate proteoglycan CSPG4 as a novel hypoxia-sensitive marker in pancreatic tumors.
Expression of the chondroitin sulphate proteoglycan, NG2, in paediatric brain tumors.
Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
Two distinct types of blood vessels in clear cell renal cell carcinoma have contrasting prognostic implications.
Schultz N, Brännström K, Byman E, Moussaud S, Nielsen HM, Netherlands Brain Bank., Olofsson A, Wennström M
Aging cell 2018 Jun;17(3):e12728
Aging cell 2018 Jun;17(3):e12728
Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Esteves CL, Sheldrake TA, Dawson L, Menghini T, Rink BE, Amilon K, Khan N, Péault B, Donadeu FX
Stem cells and development 2017 Jul 1;26(13):964-972
Stem cells and development 2017 Jul 1;26(13):964-972
Isolation and characterization of equine native MSC populations.
Esteves CL, Sheldrake TA, Mesquita SP, Pesántez JJ, Menghini T, Dawson L, Péault B, Donadeu FX
Stem cell research & therapy 2017 Apr 18;8(1):80
Stem cell research & therapy 2017 Apr 18;8(1):80
Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro.
Higgins SC, Fillmore HL, Ashkan K, Butt AM, Pilkington GJ
Anticancer research 2015 Jan;35(1):77-84
Anticancer research 2015 Jan;35(1):77-84
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
Davaine JM, Quillard T, Brion R, Lapérine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan MÁ, Charrier C, Heymann D, Gouëffic Y, Heymann MF
PloS one 2014;9(9):e107642
PloS one 2014;9(9):e107642
Chondroitin sulfate proteoglycan CSPG4 as a novel hypoxia-sensitive marker in pancreatic tumors.
Keleg S, Titov A, Heller A, Giese T, Tjaden C, Ahmad SS, Gaida MM, Bauer AS, Werner J, Giese NA
PloS one 2014;9(6):e100178
PloS one 2014;9(6):e100178
Expression of the chondroitin sulphate proteoglycan, NG2, in paediatric brain tumors.
Higgins SC, Bolteus AJ, Donovan LK, Hasegawa H, Doey L, Al Sarraj S, King A, Ashkan K, Roncaroli F, Fillmore HL, Pilkington GJ
Anticancer research 2014 Dec;34(12):6919-24
Anticancer research 2014 Dec;34(12):6919-24
Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
Benner EJ, Luciano D, Jo R, Abdi K, Paez-Gonzalez P, Sheng H, Warner DS, Liu C, Eroglu C, Kuo CT
Nature 2013 May 16;497(7449):369-73
Nature 2013 May 16;497(7449):369-73
Two distinct types of blood vessels in clear cell renal cell carcinoma have contrasting prognostic implications.
Yao X, Qian CN, Zhang ZF, Tan MH, Kort EJ, Yang XJ, Resau JH, Teh BT
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jan 1;13(1):161-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jan 1;13(1):161-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NG2/MCSP in Human Skin Cancer Tissue. NG2/MCSP was detected in immersion fixed paraffin-embedded sections of human skin cancer tissue (melanoma) using 10 µg/mL Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
