PAB28602

antibody from Abnova Corporation

Targeting: FRMD8 FKSG44, FLJ90369, iTAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28602

Provider

Abnova Corporation

Product name

FRMD8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant FRMD8.

Antigen sequence

SREKHVLLGLRFQELSWDHTSPEEEEPILWLEFDG
DSEGTPVNKLLKIYSKQAELMSSLIEYCIELSQAA
EPAGPQDSATGSPSDPSSSLAPVQRPKLRRQGSVV
SSRIQHLSTIDYVED


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate); Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with FRMD8 polyclonal antibody (Cat#PAB28602).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with FRMD8 polyclonal antibody (Cat#PAB28602) at 4 ug/ml shows positivity in nucleus but not nucleoli, plasma membrane and centrosome.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with FRMD8 polyclonal antibody (Cat#PAB28602) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)