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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-83884 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83884, RRID:AB_11047118
- Product name
- Rabbit Polyclonal FRMD8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human FRMD8 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FRMD8 Antibody [NBP1-83884] - Analysis in control (vector only transfected HEK293T lysate) and FRMD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FRMD8 Antibody [NBP1-83884] - Lane 1: HEK293T wild-type lysate (25 ug). Lane 2: HEK293T cells overexpressing C-terminally V5-tagged FRMD8 (25 ug). Customer recommends to use a dilution of 1:250 - 1:500 for the detection of overexpressed FRMD8 by Western Blot. Customer states the antibody works well for overexpressed FRMD8. This image was submitted via customer Review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FRMD8 Antibody [NBP1-83884] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
