Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-87730 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87730, RRID:AB_11014110
- Product name
- Rabbit Polyclonal Methionyl tRNA synthetase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Methionyl tRNA synthetase antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Rare recessive loss-of-function methionyl-tRNA synthetase mutations presenting as a multi-organ phenotype.
van Meel E, Wegner DJ, Cliften P, Willing MC, White FV, Kornfeld S, Cole FS
BMC medical genetics 2013 Oct 8;14:106
BMC medical genetics 2013 Oct 8;14:106
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Methionyl tRNA synthetase Antibody [NBP1-87730] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Methionyl tRNA synthetase Antibody [NBP1-87730] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of mouse olfactory bulb shows cytoplasmic positivity in mitral and external plexiform layer neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of mouse cerebral peduncle shows cytoplasmic positivity in red nucleus neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of mouse cerebellum shows cytoplasmic immunoreactivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of mouse visual cortex shows cytoplasmic and axonal staining in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of mouse pons shows neuronal positivity in motor trigeminal nucleus.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of human cerebral cortex shows cytoplasmic positivity in subsets of neurons.