AF2605

antibody from Novus Biologicals

Targeting: GATA3 HDR

Provider product page for AF2605

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [12]
Comments [0]
Validations
Western blot [2]
Immunohistochemistry [2]

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Product number: AF2605 - Provider product page
Provider: Novus Biologicals
Product name: Goat Polyclonal GATA-3 Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human and mouse GATA-3 in direct ELISAs and Western blots.
Reactivity: Human
Host: Goat
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

GATA3 protein structure - AF2605 shown in red.

Submitted references Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro.

Nomura S, Takahashi H, Suzuki J, Kuwahara M, Yamashita M, Sawasaki T
Scientific reports 2019 Nov 22;9(1):17335

Tubuloids derived from human adult kidney and urine for personalized disease modeling.

Schutgens F, Rookmaaker MB, Margaritis T, Rios A, Ammerlaan C, Jansen J, Gijzen L, Vormann M, Vonk A, Viveen M, Yengej FY, Derakhshan S, de Winter-de Groot KM, Artegiani B, van Boxtel R, Cuppen E, Hendrickx APA, van den Heuvel-Eibrink MM, Heitzer E, Lanz H, Beekman J, Murk JL, Masereeuw R, Holstege F, Drost J, Verhaar MC, Clevers H
Nature biotechnology 2019 Mar;37(3):303-313

Evaluation of variability in human kidney organoids.

Phipson B, Er PX, Combes AN, Forbes TA, Howden SE, Zappia L, Yen HJ, Lawlor KT, Hale LJ, Sun J, Wolvetang E, Takasato M, Oshlack A, Little MH
Nature methods 2019 Jan;16(1):79-87

Trophoblast organoids as a model for maternal-fetal interactions during human placentation.

Turco MY, Gardner L, Kay RG, Hamilton RS, Prater M, Hollinshead MS, McWhinnie A, Esposito L, Fernando R, Skelton H, Reimann F, Gribble FM, Sharkey A, Marsh SGE, O'Rahilly S, Hemberger M, Burton GJ, Moffett A
Nature 2018 Dec;564(7735):263-267

GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis.

Shahi P, Wang CY, Chou J, Hagerling C, Gonzalez Velozo H, Ruderisch A, Yu Y, Lai MD, Werb Z
Oncogene 2017 Oct 5;36(40):5567-5575

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.

Takasato M, Er PX, Chiu HS, Maier B, Baillie GJ, Ferguson C, Parton RG, Wolvetang EJ, Roost MS, Chuva de Sousa Lopes SM, Little MH
Nature 2015 Oct 22;526(7574):564-8

Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta.

Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar LM, Pereira L
Journal of virology 2015 May;89(9):5134-47

A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing.

Yu WM, Appler JM, Kim YH, Nishitani AM, Holt JR, Goodrich LV
eLife 2013 Dec 10;2:e01341

Cytokines inhibit norepinephrine transporter expression by decreasing Hand2.

Pellegrino MJ, Parrish DC, Zigmond RE, Habecker BA
Molecular and cellular neurosciences 2011 Mar;46(3):671-80

Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.

Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
The Journal of biological chemistry 2009 Oct 16;284(42):29109-24

Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.

Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1491-8

Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.

Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 15;181(2):1491-8

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of Human and Mouse GATA-3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GATA-3 full length (FL) at approximately 55 kDa and the splice form (SF) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of Human GATA-3 by Simple Western^TM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA-3 full length (FL) at approximately 62 kDa and the splice form (SF) at approximately 52 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: GATA-3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: GATA-3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.