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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- GTX127972 - Provider product page
- Provider
- GeneTex
- Product name
- HMGB1/HMGB2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody (GTX127972) diluted at 1:20000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB2 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extractE. 30 ?g A375 whole cell lysate/extract12 % SDS-PAGEHMGB1/HMGB2 antibody (GTX127972) dilution: 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody (GTX127972) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody (GTX127972) diluted at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody (GTX127972) diluted at 1:20000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody (GTX127972) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody (GTX127972) diluted at 1:2000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB2 protein at nucleus on human duodenal adenocarcinoma tissue by immunohistochemical analysis. Sample: Paraffin-embedded human duodenal adenocarcinoma tissue. HMGB1/HMGB2 antibody (GTX127972) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1/HMGB2 antibody detects HMGB2 protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HMGB1/HMGB2 antibody (GTX127972) dilution: 1:500.
