RQ7973

antibody from NSJ Bioreagents

Targeting: DAPK3 ZIP, ZIPK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7973

Provider

NSJ Bioreagents

Product name

Dapk3 Antibody / Death-associated protein kinase 3 / Zip kinase

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Death-associated protein kinase 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse lung, 2) mouse stomach, 3) rat kidney and 4) rat PC-12 cell lysate with Death-associated protein kinase 3 antibody. Predicted molecular weight ~51 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human RM-1 cells with Death-associated protein kinase 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse HEPA1-6 cells with Death-associated protein kinase 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Death-associated protein kinase 3 antibody.