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Learn14120-1-AP
antibody from Invitrogen Antibodies
Targeting: TPP2
TPPII
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 14120-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPPII Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LERLKDLPF IVSHRLSNTL SLDIHENHSF ALLGKKKSSN LTLPPKYNQP FFVTSLPDDK IPKGAGPGCY LAGSLTLSKT ELGKKADVIP VHYYLIPPPT KTKNGSKDKE KDSEKEKDLK EEFTEALRDL KIQWMTKLDS SDIYNELKET YPNYLPLYVA RLHQLDAEKE RMKRLNEIVD AANAVISHID QTALAVYIAM KTDPRPDAAT IKNDMDKQKS TLVDALCRKG CALADHLLHT QAQDGAISTD AEGKEEEGES PLDSLAETFW ETTKWTDLFD NKVLTFAYKH ALVNKMYGRG LKFATKLVEE KPTKENWKNC IQLMKLLGWT HCASFTENWL PIMYPPDYCV F (900-1249 aa encoded by BC039905)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of TPPII antibody (14120-1-AP, 1:1000) with si-control and si-TPPII transfected Jurkat cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14120-1-AP (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using TPP2 antibody 14120-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using TPP2 antibody 14120-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14120-1-AP ( TPPII antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 14120-1-AP ( TPPII antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug TPPII antibody (14120-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TPPII (IP:14120-1-AP, 4ug; Detection:66017-1-IG, 1:500) with Jurkat cells lysate 2400ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TPPII (IP:14120-1-AP, 4 ug; Detection:14120-1-AP 1:500) with Jurkat cells lysate 2400 ug.
