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Learn13364-1-AP
antibody from Invitrogen Antibodies
Targeting: LMAN1
ERGIC-53, ERGIC53, F5F8D, FMFD1, gp58, MCFD1, MR60
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 13364-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LMAN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GDPAVALPH RRFEYKYSFK GPHLVQSDGT VPFWAHAGNA IPSSDQIRVA PSLKSQRGSV WTKTKAAFEN WEVEVTFRVT GRGRIGADGL AIWYAENQGL EGPVFGSADL WNGVGIFFDS FDNDGKKNNP AIVIIGNNGQ IHYDHQNDGA SQALASCQRD FRNKPYPVRA KITYYQNTLT VMINNGFTPD KNDYEFCAKV ENMIIPAQGH FGISAATGGL ADDHDVLSFL TFQLTEPGKE PPTPDKEISE KEKEKYQEEF EHFQQELDKK KEEFQKGHPD LQGQPAEEIF ESVGDRELRQ VFEGQNRIHL EIKQLNRQLD MILDEQRRYV SSLTEEISKR GAGMPGQHGQ ITQQELDTVV KTQHEILR (35-401 aa encoded by BC032330 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed A549 cells using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ERGIC-53 (IP:13364-1-AP, 4ug; Detection:13364-1-AP 1:400) with HepG2 cells lysate 1000 ug.
