Explore
Validate
LearnNBP2-03381
antibody from Novus Biologicals
Targeting: LMAN1
ERGIC-53, ERGIC53, F5F8D, FMFD1, gp58, MCFD1, MR60
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-03381 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LMAN1 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Ceramide phosphoethanolamine synthase SMSr is a target of caspase-6 during apoptotic cell death.
Rab18 and a Rab18 GEF complex are required for normal ER structure.
Cabukusta B, Nettebrock NT, Kol M, Hilderink A, Tafesse FG, Holthuis JCM
Bioscience reports 2017 Aug 31;37(4)
Bioscience reports 2017 Aug 31;37(4)
Rab18 and a Rab18 GEF complex are required for normal ER structure.
Gerondopoulos A, Bastos RN, Yoshimura S, Anderson R, Carpanini S, Aligianis I, Handley MT, Barr FA
The Journal of cell biology 2014 Jun 9;205(5):707-20
The Journal of cell biology 2014 Jun 9;205(5):707-20
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staurosporine-induced cleavage of SMSr is sensitive to caspase-6 inhibitors. HeLa cells stably transduced with EV, SMSr-V5, and SMSrCR-V5 were treated with 1 ug/ml staurosporine for 6 h, lysed and subjected to immunoblot analysis using anti-V5, anti-PARP1, and anti-ERGIC-53 antibodies; FL, full length; CL, cleaved. Image collected and cropped by CiteAb from the following publication (http://bioscirep.org/lookup/doi/10.1042/BSR20170867), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Analysis of Jurkat cells, using anti-LMAN1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381] - HEK293T cells transfected with either RC207088 overexpress plasmid(Red) or empty vector control plasmid(Blue) wereimmunostained by anti-LMAN1 antibodyand then analyzed by flow cytometry.
