21741-1-AP

antibody from Invitrogen Antibodies

Targeting: CARD11 BIMP3, CARMA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21741-1-AP

Provider

Invitrogen Antibodies

Product name

CARD11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DTMYQDRHE WLCARVDPFT DHDLDMGTIP SYSRAQQLLL VKLQRLMHRG SREEVDGTHH TLRALRNTLQ PEEALSTSDP RVSPRLSRAS FLFGQLLQFV SRSENKYKRM NSNERVRIIS GSPLGSLARS SLDATKLLTE KQEELDPESE LGKNLSLIPY SLVRAFYCER RRPVLFTPTV LAKTLVQRLL NSGGAMEFTI CKSDIVTRDE FLRRQKTETI IYSREKNPNA FECIAPANIE AVAAKNKHCL LEAGIGCTRD LIKSNIYPIV LFIRVCEKNI KRFRKLLPRP ETEEEFLRVC RLKEKELEAL PCLYATVEPD MWGSVEELLR VVKDKIGEEQ RKTIWVDEDQ L (805-1154 aa encoded by BC111719)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 21741-1-AP ( CARD11 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed L02 cells using CARD11 antibody (Product # 21741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of L02 cells using 21741-1-AP (CARD11 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).