Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF3080 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal A33/GPA33 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- A33 in Human Colon. A33 was detected in immersion fixed paraffin-embedded sections of human colon using 1.7 µg/mL Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of A33 in TH-29 Human Cell Line by Flow Cytometry. TH-29 human colon carcinoma cell line was stained with Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).