MA5-42830

antibody from Invitrogen Antibodies

Targeting: GPA33 A33

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42830

Provider

Invitrogen Antibodies

Product name

A33 Recombinant Rabbit Monoclonal Antibody (9W5A7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: HCT116, HT-29, A-549, Mouse stomach, Rat large intestine. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

9W5A7

Vial size

100 µL

Concentration

0.23 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of A33 in various cell lines using a A33 Recombinant Monoclonal antibody (Product # MA5-42830) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1 min.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of A33 in human colon carcinoma using a A33 Recombinant Monoclonal antibody (Product # MA5-42830). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of A33 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42830) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..