Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F55008 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GPA33 Antibody / Cell surface A33 antigen
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.08 µl, 0.4 µl
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the GPA33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) 293T and 2) HT-29 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) HEK293 and 3) MDA-MB-231 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human colon carcinoma tissue with GPA33 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human MDA-MB-231 cells with GPA33 antibody; Blue=isotype control, Green= GPA33 antibody.