- 40-8900 - Invitrogen Antibodies JAM3 antibody

Submitted references Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.

Qian X, Mruk DD, Cheng CY
PloS one 2013;8(4):e60656

Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes.

Qian X, Mruk DD, Wong EW, Lie PP, Cheng CY
Endocrinology 2013 May;154(5):1907-20

Breast cancer resistance protein regulates apical ectoplasmic specialization dynamics stage specifically in the rat testis.

Qian X, Mruk DD, Wong EW, Cheng CY
American journal of physiology. Endocrinology and metabolism 2013 Apr 1;304(7):E757-69

Evidence for cross-reactivity of JAM-C antibodies: implications for cellular localization studies.

Betanzos A, Schnoor M, Severson EA, Liang TW, Parkos CA
Biology of the cell 2009 Jun 4;101(8):441-53

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of (A) TCMK-1 cell lysates, (B) mouse heart and (C) kidney homogenates, (D) EL-4, (E) A375, (F) MeWo, and (G) NGP96 cell lysates using Zymed Rb anti-JAM-C (C-term) (Product # 40-8900).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of JAM-C was performed by loading 20 µg of Mouse Placenta (lane1), Mouse Testis (lane2), Jurkat (lane3) U2OS (lane4) and SH-SY5Y (lane6) lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5 % skim milk for 1 hour at room temperature. JAM-C was detected at ~45 kDa using JAM-C Rabbit Polyclonal Antibody (Product # 40-8900) at 1-2 µg/mL in 5 % skim milk at 4ºC overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of (A) TCMK-1 cell lysates, (B) mouse heart and (C) kidney homogenates, (D) EL-4, (E) A375, (F) MeWo, and (G) NGP96 cell lysates using Zymed Rb anti-JAM-C (C-term) (Product # 40-8900).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of JAM-C was performed by loading 20 µg of Mouse Placenta (lane1), Mouse Testis (lane2), Jurkat (lane3) U2OS (lane4) and SH-SY5Y (lane6) lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5 % skim milk for 1 hour at room temperature. JAM-C was detected at ~45 kDa using JAM-C Rabbit Polyclonal Antibody (Product # 40-8900) at 1-2 µg/mL in 5 % skim milk at 4ºC overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of JAM-C showing staining in the membrane of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a JAM-C polyclonal antibody (Product # 40-8900) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Rai14 is an actin-binding protein in the rat testis. ( A ) A study by RT-PCR to confirm the expression of Rai14 in adult rat testis, Sertoli cells (SC, isolated from 20-day-old rat testes and cultured for 4-day), and germ cells (GC, isolated from adult rat testes and cultured for 16 hr). ( B ) Immunoblotting also confirmed the expression of Rai14 in the rat testis, Sertoli and germ cells, and the relative expression of Rai14 in SC vs. GC was shown in the histogram with n = 3 experiments in which the relative expression level of Rai14 in the testis was arbitrarily set at 1 so that the relative expression level between these samples can be compared. ( C ) The specificity of the anti-Rai14 antibody ( Table 1 ) was assessed by immunoblotting using lysates of GC (20 ug protein). ( D ) Using the specific anti-Rai14 antibody, Rai14 was shown to be an actin-binding protein by co-immunoprecipitation (Co-IP); however, Rai14 did not structurally interact with any of the BTB-associated proteins including several actin-binding and regulatory proteins ( e.g ., Arp3, drebrin E, Eps8) and vimentin (an intermediate filament-based constituent protein). However, Rai14 was found to structurally interact with an actin cross-linking protein palladin which is known to be involved in conferring actin filament bundles in other mammalian cells [48] . ( E ) Rai14 (red) was also shown to be an actin-binding protein by dual-labeled immunofluorescence analysis in which it co-localized with F-acti

40-8900

Antibody data