Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 41-3239-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD323 (JAM3) Monoclonal Antibody (SHM33), eFluor 570, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SHM33 monoclonal antibody reacts with human CD323, which is also known as Junctional Adhesion Molecule (JAM) 3. A member of the Ig superfamily of transmembrane proteins, CD323 is expressed on endothelial and epithelial cells, smooth muscle cells, peripheral nerves, platelets, and some B cells. In addition, this receptor is expressed on VEGF and histamine-stimulated endothelial cells, as well as on activated T cells. CD323 is involved in neutrophil transmigration during inflammation, angiogenesis, cell polarity, and nerve conduction. CD323 interacts homo- and heterotypically with beta2 and beta3 integrins, JAM2, and the viral receptor CAR. Applications Reported: This SHM33 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This SHM33 antibody has been tested by immunocytochemistry of methanol-fixed human cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SHM33
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Transcriptional induction of junctional adhesion molecule-C gene expression in activated T cells.
Genetic deletion of JAM-C reveals a role in myeloid progenitor generation.
JAM-C regulates tight junctions and integrin-mediated cell adhesion and migration.
Junctional adhesion molecule-C regulates the early influx of leukocytes into tissues during inflammation.
Immenschuh S, Naidu S, Chavakis T, Beschmann H, Ludwig RJ, Santoso S
Journal of leukocyte biology 2009 May;85(5):796-803
Journal of leukocyte biology 2009 May;85(5):796-803
Genetic deletion of JAM-C reveals a role in myeloid progenitor generation.
Praetor A, McBride JM, Chiu H, Rangell L, Cabote L, Lee WP, Cupp J, Danilenko DM, Fong S
Blood 2009 Feb 26;113(9):1919-28
Blood 2009 Feb 26;113(9):1919-28
JAM-C regulates tight junctions and integrin-mediated cell adhesion and migration.
Mandicourt G, Iden S, Ebnet K, Aurrand-Lions M, Imhof BA
The Journal of biological chemistry 2007 Jan 19;282(3):1830-7
The Journal of biological chemistry 2007 Jan 19;282(3):1830-7
Junctional adhesion molecule-C regulates the early influx of leukocytes into tissues during inflammation.
Aurrand-Lions M, Lamagna C, Dangerfield JP, Wang S, Herrera P, Nourshargh S, Imhof BA
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6406-15
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6406-15
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of methanol-fixed HEK293 cells stained with 1 µg/mL of Mouse IgG2a K Isotype Control eFluor® 570 (left) or 1 µg/mL of Anti-Human CD323 (JAM3) eFluor® 570 (right). Nuclei are stained with DAPI.