AF1189

antibody from R&D Systems

Targeting: JAM3 JAM-C, JAMC

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1189

Provider

R&D Systems

Product name

Human JAM-C Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human JAM-C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse (rm) JAM-C is observed and less than 1% cross-reactivity with recombinant human JAM-1 and rmJAM-1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9BX67


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.

Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schön MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
Immunology 2009 Oct;128(2):196-205

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Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.

Fuse C, Ishida Y, Hikita T, Asai T, Oku N
The Journal of biological chemistry 2007 Mar 16;282(11):8276-83

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JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration.

Zen K, Babbin BA, Liu Y, Whelan JB, Nusrat A, Parkos CA
Molecular biology of the cell 2004 Aug;15(8):3926-37

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human JAM-C by Simple Western^TM. Simple Western lane view shows lysates of JAR human choriocarcinoma cell line and human placenta, loaded at 0.2 mg/mL. A specific band was detected for JAM-C at approximately 53 and 54 kDa (as indicated) using 20 µg/mL of Goat Anti-Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human JAM-C by Western Blot. Western blot shows lysates of human placenta tissue, human brain tissue, JAR human choriocarcinoma cell line, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for JAM-C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Adhesion Mediated by JAM-C and Neutralization by Human JAM-C Antibody. Recombinant Human JAM-B/ VE-JAM Fc Chimera (Catalog # 1074-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189). The ND₅₀ is typically 2-8 µg/mL.