ACR-042-25UL

antibody from Invitrogen Antibodies

Targeting: CCKBR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ACR-042-25UL

Provider

Invitrogen Antibodies

Product name

CCKBR (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200).3,4. Anti-CCKBR (extracellular) Antibody , preincubated with CCKBR (extracellular) Blocking Peptide (#BLP-CR042).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200).3,4. Anti-CCKBR (extracellular) Antibody , preincubated with CCKBR (extracellular) Blocking Peptide (#BLP-CR042).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of CCKBR in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-CCKBR (extracellular) Antibody   (#ACR-042), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus region, showed immunoreactivity (green) in the granule layer (G, vertical arrows) and in interneurons (horizontal arrows). B. Pre-incubation of the Antibody with CCKBR (extracellular) Blocking Peptide (#BLP-CR042), suppressed staining. Cell nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of CCKBR in rat hypothalamus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-CCKBR (extracellular) Antibody   (#ACR-042), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the rat dorsomedial hypothalamus region, showed immunoreactivity (green) in neurons (vertical arrows) and along the wall of 3rd ventricle (horizontal arrow). B. Pre-incubation of the Antibody with CCKBR (extracellular) Blocking Peptide (#BLP-CR042), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Cholecystokinin B receptor in rat stomach - Immunohistochemical staining of paraffin embedded rat stomach sections using Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:100). Cholecystokinin B receptor (brown) is expressed in both parietal cells (black arrows) and in chief cells (red arrows) of the gastric mucosa. Hematoxilin is used as the counterstain.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of Cholecystokinin B receptor by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-PE. (green) Cells + Anti-CCKBR (extracellular) Antibody (#ACR-042), (5μg) + goat- Anti-rabbit-PE.