Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2654 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXADR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CXADR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant human CXADR is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P97792
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Coxsackievirus Adenovirus Receptor Loss Impairs Adult Neurogenesis, Synapse Content, and Hippocampus Plasticity.
A pRb-responsive, RGD-modified, and hyaluronidase-armed canine oncolytic adenovirus for application in veterinary oncology.
Enhanced adenoviral gene delivery to motor and dorsal root ganglion neurons following injection into demyelinated peripheral nerves.
Zussy C, Loustalot F, Junyent F, Gardoni F, Bories C, Valero J, Desarménien MG, Bernex F, Henaff D, Bayo-Puxan N, Chen JW, Lonjon N, de Koninck Y, Malva JO, Bergelson JM, di Luca M, Schiavo G, Salinas S, Kremer EJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Sep 14;36(37):9558-71
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Sep 14;36(37):9558-71
A pRb-responsive, RGD-modified, and hyaluronidase-armed canine oncolytic adenovirus for application in veterinary oncology.
Laborda E, Puig-Saus C, Rodriguez-García A, Moreno R, Cascalló M, Pastor J, Alemany R
Molecular therapy : the journal of the American Society of Gene Therapy 2014 May;22(5):986-98
Molecular therapy : the journal of the American Society of Gene Therapy 2014 May;22(5):986-98
Enhanced adenoviral gene delivery to motor and dorsal root ganglion neurons following injection into demyelinated peripheral nerves.
Zhang Y, Zheng Y, Zhang YP, Shields LB, Hu X, Yu P, Burke DA, Wang H, Jun C, Byers J, Whittemore SR, Shields CB
Journal of neuroscience research 2010 Aug 15;88(11):2374-84
Journal of neuroscience research 2010 Aug 15;88(11):2374-84
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CXADR by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. Specific bands were detected for CXADR at approximately 60 and 53 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse CXADR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2654) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CXADR by Western Blot. Western blot shows lysates of mouse liver tissue and mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CXADR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CXADR at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.