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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-110995 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EVHHDIREDV PPPKSRTSTA RSYIGSNHSS LGSMSPSNME GYSKTQYNQV PSEDFERTPQ SPTLPPAKVA APNLS
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.15 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAR in human cell lines Caco-2 and MCF-7 using CAR Polyclonal Antibody (Product # PA5-110995). Corresponding CAR RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CAR in human cell line U-2 OS using CAR Polyclonal Antibody (Product # PA5-110995) shows localization to nucleoplasm, plasma membrane and cell junctions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CAR in human rectum and skeletal muscle tissues using CAR Polyclonal Antibody (Product # PA5-110995). Corresponding CAR RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CAR in human rectum tissue using CAR Polyclonal Antibody (Product # PA5-110995). Staining shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CAR in human skeletal muscle tissue using CAR Polyclonal Antibody (Product # PA5-110995). Staining shows low expression as expected.
