MA5-32373

antibody from Invitrogen Antibodies

Targeting: RUNX3 AML2, CBFA3, PEBP2A3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32373

Provider

Invitrogen Antibodies

Product name

RUNX1/RUNX2/RUNX3 Recombinant Rabbit Monoclonal Antibody (SD0803)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

SD0803

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RUNX1/RUNX2/RUNX3 in Jurkat cell lysates using a RUNX1/RUNX2/RUNX3 Monoclonal antibody (Product # MA5-32373) at a dilution of 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RUNX1/RUNX2/RUNX3 in N2A cells using a RUNX1/RUNX2/RUNX3 Monoclonal antibody (Product # MA5-32373) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RUNX1/RUNX2/RUNX3 in MCF-7 cells using a RUNX1/RUNX2/RUNX3 Monoclonal antibody (Product # MA5-32373) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RUNX1/RUNX2/RUNX3 in SHG-44 cells using a RUNX1/RUNX2/RUNX3 Monoclonal antibody (Product # MA5-32373) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RUNX1/RUNX2/RUNX3 of paraffin-embedded Mouse testis tissue using a RUNX1-RUNX2-RUNX3 Monoclonal antibody (Product #MA5-32373). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RUNX1/RUNX2/RUNX3 of paraffin-embedded Human tonsil tissue using a RUNX1-RUNX2-RUNX3 Monoclonal antibody (Product #MA5-32373). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of RUNX1/RUNX2/RUNX3 in Jurkat cells using a RUNX1/RUNX2/RUNX3 Monoclonal Antibody (Product # MA5-32373) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.