Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 676102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#676102, RRID:AB_2565610
- Product name
- Purified anti-SATB1
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant human SATB1 (NP_002962) produced in HEK293T cells.
- Description
- SATB1 is a global regulator that selectively tethers multiple genomic loci and recruits chromatin-remodeling enzymes (e.g. ACF, ISWI, HDAC1 and histone acetyltransferase) to control chromatin structure and gene expression. SATB1 also recruits corepressors or coactivators to promoters. These two opposing interactions, however, are determined by whether or not the SATB1 is phosphorylated by protein kinase C. Unphosphorylated and phosphorylated SATB1 preferably binds to PCAF and HDAC1, respectively. SATB1 also forms a regulatory complex with the promyelocytic leukaemia (PML) protein to organize the major histocompatibility complex (MHC) class I locus into distinct chromatin-loop architecture and mediate the expression of a subset of MHC class I genes. Upon Th2 cell activation, SATB1 expression is induced to compact chromatin into dense-loop structure and to prompt the expression of IL-4, IL-5, IL-13 and c-Maf. Research found that SATB1 is crucial for the growth and metastasis of breast cancer and is a potential prognostic marker.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- O96C6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from Jurkat (lane 1, 15 µg), mouse thymus (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-SATB1 (clone O96C6) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.
- Conjugate
- Unconjugated