Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 676104 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#676104, RRID:AB_2566180
- Product name
- Alexa Fluor® 594 anti-SATB1
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant human SATB1 (NP_002962) produced in HEK293T cells.
- Description
- SATB1 is a global regulator that selectively tethers multiple genomic loci and recruits chromatin-remodeling enzymes (e.g. ACF, ISWI, HDAC1 and histone acetyltransferase) to control chromatin structure and gene expression. SATB1 also recruits corepressors or coactivators to promoters. These two opposing interactions, however, are determined by whether or not the SATB1 is phosphorylated by protein kinase C. Unphosphorylated and phosphorylated SATB1 preferably binds to PCAF and HDAC1, respectively. SATB1 also forms a regulatory complex with the promyelocytic leukaemia (PML) protein to organize the major histocompatibility complex (MHC) class I locus into distinct chromatin-loop architecture and mediate the expression of a subset of MHC class I genes. Upon Th2 cell activation, SATB1 expression is induced to compact chromatin into dense-loop structure and to prompt the expression of IL-4, IL-5, IL-13 and c-Maf. SATB1 is also reported as being crucial for the growth and metastasis of breast cancer and is a potential prognostic marker.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- O96C6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat cells were fixed with 1% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-SATB1 antibody (clone O96C6, red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue).
- Conjugate
- Yellow dye